M.C.I. Santé AnimaleM.C.I. Santé Animale
  • Home
  • About Us
    • HISTORY
    • EUROPEAN STANDARDS
    • VALUES
  • RESEARCH AND DEVELOPMENT
    • SCIENTIFIC PUBLICATIONS
  • PRODUCTS
    • M.C.I SANTE ANIMALE RANGE
      • AVIAN
      • RUMINANTS
      • PETS
      • EQUINES
    • DISTRIBUTED LINE IN MOROCCO
      • AVIAN
      • RUMINANTS
      • PETS
      • EQUINES
  • CONTACT US
    • OUR REPRESENTATIVES
  • CAREERS
Search
  • FR
  • Home
  • About Us
    • HISTORY
    • EUROPEAN STANDARDS
    • VALUES
  • RESEARCH AND DEVELOPMENT
    • SCIENTIFIC PUBLICATIONS
  • PRODUCTS
    • M.C.I SANTE ANIMALE RANGE
      • AVIAN
      • RUMINANTS
      • PETS
      • EQUINES
    • DISTRIBUTED LINE IN MOROCCO
      • AVIAN
      • RUMINANTS
      • PETS
      • EQUINES
  • CONTACT US
    • OUR REPRESENTATIVES
  • CAREERS

Vaccination Against Hydatidosis: Molecular Cloning and Optimal Expression of the EG95NC− Recombinant Antigen in Escherichia coli

M. Jazouli, M. Lightowlers, G. Gauci, K. Tadlaoui, A. Belmlih, M. M. Ennaji, M. Elharrak

Cystic echinococcosis (CE) is a widely distributed zoonosis that is highly endemic in the Mediterranean basin. The disease represents a serious public health threat and causes economic losses. The parasite life-cycle involves dogs and ruminants as definitive and intermediate hosts; humans are accidently infected, causing serious clinical issues. Vaccination of ruminants and dog treatments represent the most efficient measures to prevent parasite transmission. The recombinant protein vaccine, EG95, has been used successfully in sheep vaccine trials against CE in several countries. In this study, we expressed the modified antigen, EG95NC-GST, in Escherichia coli for use as a vaccine against Echinococcus granulosus in ruminants. We tested three different media formulations for E. coli culture and established for each culture conditions for optimal levels of soluble EG95 expression. The results demonstrate that SOC and TB media provided high yields in cell density and EG95 protein expression. Purification of the recombinant protein with affinity chromatography (using FPLC) was also performed to increase the purity of the EG95NC−-GST antigen.

Keywords : Echinococcus granulosus · Hydatidosis · Escherichia coli · Recombinant protein · Vaccine

Disease: Hydatidosis
Published: 14 Novembre 2017

DOWNLOAD PUBLICATION

Contact us


PHONE

  • +212 523 30 31 32 (LG)
  • +212 523 30 21 33
  • +212 523 30 21 29
  • Fax : +212 523 30 21 30

EMAIL

contact@mci-santeanimale.com

Useful links


  • About us
  • M.C.I santé animale product range
  • Product distributed in Morocco
  • News
  • Privacy Policy

Opening hours


  • Mon - Fri: 9:00 am to 6:00 pm
  • GMT+1

ADDRESS

Lot. 157, Zone Industrielle Sud-Ouest (ERAC) B.P : 278 Mohammedia 28810 MOROCCO

M.C.I Santé Animale © 2022. All Rights Reserved.

Subscribe to our newsletter